Background: Urinary tract infection (UTI) is a common health hazard among pregnant w omen .UTI may manifests either as a symptomatic bacteriuria or asymptomatic bacteriuria. As per the authors, the prevalence of asymptomatic UTI is 2% to 10% in pregnant women while symptomatic UTI accounts for 1-18% during pregnancy. Objective: The Objective of Study is to detect significant bacteriuria in pregnant women during antenatal period & to find out Antibiotic susceptibility and resistance pattern of various bacterial isolates from urine sample of antenatal patients and establishing its relationship with presence or absence of symptoms. Methodology: This hospital based prospective study was carried out on urine samples of 100 pregnant women to determine the profile of bacteria and their susceptibility pattern isolated from pregnant women with and without symptoms of Urinary tract infection (UTI). Mid-stream urine samples were collected using sterile, wide mouthed container with screw cap top. Predesigned and structured questionnaire was used for the collection of data. Urine samples were processed in the laboratory within 2 hours of collection. Urine sample was examined for Gross Examination, Wet mount examination, Gram staining, Culture and Colony counting and Antibiotic Sensitivity Test.