Some normal flora fungi can cause infection if there is a disruption in the protective barrier of the skin and mucous membranes or a defect in the immune system that allows them to enter, colonize and multiply in the body. Many fungal pathogens can produce subcutaneous lesions, given the complexity of patients at risk of infection and the variety of fungal pathogens. Direct microscopy is essential to detect fungi for appropriate treatment and patient recovery. This study aims to see the picture of staining between Hematoxylin Eosin (HE) with Periodic Acid Schiff (PAS) McManus to identify Subcutaneous mycoses fungi on histopathology tissue pieces. This study is a descriptive observational study with a cross-sectional design. The number of samples was total sampling. Hematoxylin Eosin (HE) and Periodic Acid Schiff (PAS) McManus staining were performed to detect Subcutaneous mycoses fungi and then read by 3 academicians with clear, less clear, and unclear categories. Based on the results of this study showed that there were 6 samples of fungal infection Subcutaneous mycoses obtained through Hematoxylin Eosin (HE) staining. Only 2 fungi were visible, and 4 fungi were less clear. In comparison, Periodic Acid Schiff (PAS) McManus staining of the 6 fungal samples was visible. In conclusion, the picture of PAS McManus staining is better than HE staining for detecting Subcutaneous mycoses fungi in histopathology tissue.