The present work concentrates on experimental investigation to evaluate the performance characteristics of a single cylinder, four stroke, water cooled diesel engine by using Calophyllum Inophyllum biodiesel blends with TiO2 nanoparticles as an additive with engine having maximum power of 3.7 kW at 1500 rpm. Experimental analysis has been carried out for Crude Diesel (baseline fuel), ratios of Biodiesel-Diesel blends and upon addition of TiO2 nanoparticles in the blends for various loading condition ranging from no-load to full-load. The TiO2 nanoparticles were dispersed into different blends fuel with a dosage of 30 ppm. From the investigation it is concluded that Calophyllum Inophyllum biodiesel blend (B10) with the addition of TiO2 nanoparticles exhibits better engine performance compared to the other fuels.