The government hospitals in Al Riyadh provide comprehensive health care services to patients, including registration, admission and follow-up procedures for patients’ health conditions, and providing appropriate treatment and medications for each disease condition, which often requires nurses and pharmacists to take immediate action. This study aimed to explore the pharmacists and nursing attitudes toward nursing, pharmacists, collaboration at government hospitals in Al Riyadh, the study was applied to (192) nurses and pharmacists in government hospitals at Al Riyadh, The results of the study showed that the average scores of nursing and pharmacists on the items of the study instrument were close, and there was no statistically significant difference in the level of their attitudes toward cooperation among themselves. The results showed that nursing and pharmacists had positive attitudes toward clinical discussions between nurses and pharmacists, which confirm that nursing is learned about the medications that the pharmacist provides to patients, but they emphasized the main role of the doctor in determining the appropriate medications for patients according to their medical condition. It can be said that there are positive trends among nurses and pharmacists in government hospitals in Riyadh towards working as a team among themselves.