The study includes pharmacognostic evaluation, phytochemical analysis, and pharmacological activity of the ethanol extract obtained from the root bark. The pharmacognostic evaluation involved the macroscopic and microscopic examination of the root bark, determining its organoleptic characters, size, shape, surface features, and other parameters. Physico-chemical standards such as loss on drying, total ash, acid-insoluble ash, water-soluble ash, and extractive values were also determined to assess the quality and purity of the root bark. Phytochemical analysis of the ethanolic and aqueous extracts revealed the presence of various phytoconstituents including carbohydrates, reducing sugars, alkaloids, glycosides, cardiac glycosides, flavonoids, triterpenoids, tannins, and steroids. These phytoconstituents are known for their medicinal properties and contribute to the therapeutic potential of the root bark. The pharmacological activity of the ethanol extract was evaluated through anticancer assays using the onion tip root assay and potato disk assay methods. The results showed significant inhibitory effects on tumor growth, indicating potential antitumor activity.Overall, this comprehensive study provides valuable insights into the pharmacognostic evaluation, phytochemical analysis, and pharmacological activity of Aegle marmelos root bark. The findings contribute to the understanding of its medicinal properties and support its potential use in pharmaceutical and natural product research.