A study was designed to determine preclinical activity of Vigna mungo seed Supercritical carbon-dioxide fluid extract and normal ethanolic extract in streptozotocin induced diabetic rats for diabetic neuropathy. The preliminary phytochemical analysis of Vigna mungo Supercritical carbon-dioxide fluid extract and normal ethanolic extract was performed by standard phytochemical procedures whereas preclinical evaluation for diabetic neuropathy activity was carried out in Streptozotocin induced diabetic rats. Materials and methods: Diabetes was induced in male Sprague Dawley rats with streptozotocin(60 mg/kg i.p) and were divided into seven groups namely Normal control, Negative Control, third and fourth test groups of Supercritical fluid extract seeds of Vigna mungo (250 mg/kg and 500 mg/kg b.w.) fifth group Standard drug Glibenclamide 5mg/kg i.p. +Metformin 25 mg/kg i.p. sixth and seventh test groups of ethanolic extract seeds of Vigna mungo (250 mg/kg and 500 mg/kg b.w.) After the 4th week of diabetes, induction treatment was started for further 28 days (5th to 8th week) with Results: Significant activity against diabetic neuropathy was shown by Supercritical fluid extract seeds of Vigna mungo. Discussion: Vigna mungo Supercritical carbon-dioxide fluid extract possess phytoconstituents with antidiabetic potentiality. Also, it offers a scientific platform and baseline information for further analysis such as isolation and standardization of its bioactive compounds as herbal alternative for diabetic complications.