Background: The successful administration of pharmacological treatments is essential for patient well-being, and healthcare professionals, especially graduating students, play a crucial role in ensuring the safe and effective use of drugs. This study seeks to assess the pharmacological knowledge and preparedness of graduating students across multiple institutions, aiming to identify areas of improvement in drug treatment practices. Aim: The primary aim of this cross-institutional analysis is to evaluate the pharmacological knowledge and preparedness of graduating students. By assessing their comprehension of drug mechanisms, dosages, adverse effects, and regulatory guidelines, we aim to identify potential gaps in their education and training. Methods: A comprehensive survey was distributed to graduating students from various healthcare programs across different institutions. The survey focused on evaluating their understanding of pharmacological concepts, clinical decision-making, and familiarity with drug interactions. Statistical analysis and comparative assessments were conducted to draw meaningful insights. Results: The analysis revealed varying levels of pharmacological knowledge and preparedness among graduating students from different institutions. While some students demonstrated a strong grasp of drug treatment principles, others exhibited deficiencies in crucial areas. Factors influencing these disparities were examined, including curriculum variations and teaching methodologies. Conclusion: This cross-institutional analysis underscores the need for consistent and standardized pharmacological education across healthcare programs. Graduating students' varying levels of preparedness may affect patient safety and the effectiveness of drug treatments. Institutions should consider implementing comprehensive pharmacological education and emphasizing the importance of evidence-based clinical decision-making to ensure safer drug administration and enhanced patient outcomes.