A detailed analysis was undertaken to determine the physico-chemical characteristics and pollution level in the sample collected from one of the distillery situated in the area of Unnao, India. The samples were collected from a fixed spot of the distillery in one year duration from Jan 2011 to Dec. 2011 at monthly interval. Presence of high chemical load like iron, calcium, magnesium, carbonate, bicarbonate, chloride, BOD, COD etc. were observed in the discharged effluent of the above stated distillery. High variations were observed according to monthly level and results showed that there are certain relationships between physico- chemical characteristics of effluents of both positive and negative.