The quality of Hindon river water has substantially declined because of unregulated discharge of waste materials without treatment, originating from industrial, domestic, and agricultural practices. Water quality monitoring is one of the most essential river protection and conservation activities. The present study was managed to access the physiochemical and microbiological parameter of Hindon river water that has been contaminated by the unwanted discharges. Between April to Dec., 2021, water samples from river Hindon were collected from the seven different sites from Saharanpur to its confluence in Yamuna River at Momnathal Village Greater Noida. The water samples were examined for multiple parameters, including Temp., pH, DO (Dissolved oxygen), Turbidity, TDS (Total Dissolved Solids), Salinity, TH (Total Hardness), COD (Chemical Oxygen Demand), BOD (Biochemical Oxygen Demand), as well as the occurrence of heavy metals such as Cd (cadmium), Ni (nickel), Mo (molybdenum), Cu (copper), As (arsenic), Cr (chromium), Pb (lead), Mn (manganese), Zn (zinc), Co (cobalt) and Fe (iron). Furthermore, the total and fecal coliform in all samples were quantified. After conducting a comprehensive study, it was found that all physicochemical and microbiological attributes have surpassed the permissible threshold compared to the BIS standard (IS-10500). Therefore, the river Hindon's water quality was found to be unfit for irrigation, fish farming, human consumption etc., based on the findings of current study, a complementary technique combining physicochemical and microbiological studies should be used to obtain a complete information of the river Hindon.