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ISSN 2063-5346
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Physicochemical, Qualitative and Quantitative Determination of Secondary Metabolites and in vitro Antioxidant Potential of Bombax ceiba Linn Flower Extracts

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Pinki Phougat, Hitesh Kumar, Neha Sharma
» doi: 10.48047/ecb/2023.12.si4.580

Abstract

Bombax ceiba Linn (Bombacaceae) is a tall tree buttressed at the base that is widely distributed throughout India, Ceylon and Malaya up to 1500 m of altitude. Many parts of the plant (root, stem bark, gum, leaf, prickles, flower, fruit, seed and heartwood) are used by various tribal communities and forest dwellers for the treatment of a variety of ailments. It also possesses important pharmacological activity such as aphrodisiac, anti-inflammatory and hepatoprotective activity in addition to anticancer and anti-HIV activity, anti-Helicobacter pylori, antiangiogenic, analgesic and antioxidant activity and hypotensive, hypoglycemic and antimicrobial activity. It is reported to contain important phytoconstituents such as naphthol, naphthoquinones, polysaccharides, anthocyanins, shamimin and lupeol. The aim of the present study was to evaluate physicochemical, qualitative and quantitative phytochemical analysis and in vitro antioxidant activities of flower of Bombax ceiba collected from local areas of Sonipat, Haryana. The different physicochemical parameters were evaluated as per standard protocols with some modifications. Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenolics and flavonoids were determined by the well-known test protocol available in the literature. Quantitative analysis of phenolic and flavonoids was carried out by Folins Ciocalteau reagent method and aluminium chloride method respectively. The In vitro antioxidant activity of methanolic extract of the flowers was assessed against DPPH and hydrogen peroxide scavenging assay method using standard protocols. Phytochemical estimation of petroleum ether extract of Bombax ceiba showed the presence of terpenoids and saponins. Flavonoids, alkaloids, carbohydrates, tannins and phenolic compounds, saponins, protein and amino acids, glycosides were detected in methanolic extractof Bombax ceiba. The total phenolics content of flowers methanolic extract was 45.75mg/100mg, followed by flavonoids 38.5mg/100mg. The activities of methanolic flower extract against DPPH and hydrogen peroxide scavenging assay method were concentration dependent. The diverse array of phytochemicals present in the plant thus suggests its therapeutic potentials which may be explored in drug manufacturing industry as well as in traditional medicine.

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