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ISSN 2063-5346
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Physiochemical analysis, antioxidant activity and phenolic content determination of Bergenia ciliata (Haw.) Sternb. Leaves

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Prashant Kumar Singh, Mohammad Mukim, Shalini Tripathi, AKS Rawat
» doi: 10.48047/ecb/2023.12.si13.112

Abstract

Many medicinal plants contain active compounds that play a crucial role in treating various diseases. People often utilize plants and herbal remedies to enhance their well-being and tap into their beneficial properties. This study aimed to investigate the physiochemical properties of a particular plant and assess the effects of different solvents (ethanol, methanol, and water) on its phenolic profile and ability to scavenge free radicals. The researchers followed standard procedures for preliminary phytochemical and physiochemical analyses. They employed the Folin-Ciocalteu method and a colorimetric approach to quantify the phytochemical content. The free radical scavenging potential was evaluated using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and 2, 2′′-azino-bis(3-ethylbenzothiazoline-6-sulphonic) acid (ABTS) assays. The results indicated that ethanol was the most effective solvent for extracting polyphenols. The quantitative phytochemical analysis revealed that the ethanol extract exhibited the highest phenolic content (73.87 mg/g GAE) and flavonoid content (86.90 mg/g QE), while the aqueous extract had the lowest phenolic content (30.33 mg/g GAE) and flavonoid content (22.32 mg/g QE). Additionally, the ethanolic extract of B. ciliata demonstrated the highest scavenging potential against DPPH (33.03 µg/mL) and ABTS (24.69 µg/mL). These findings suggest that the ethanolic extract of B. ciliata leaves possesses significant antioxidant properties and has the potential to serve as a valuable source of organic antioxidants for the development of functional foods.

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