The first planned city with properly managed plantations and green space is Chandigarh. In light of growing urbanisation, herbs or medicinal plants found in urban green spaces are important. In the present study, eight medicinal plants such as Asparagus racemosus, Asparagus officinalis, Murraya koenigii, Murraya paniculata, Withania somnifera, Centella asiatica, Cinnamomum tamala, and Glycyrrhiza glabara from the urban green space of Chandigarh were analysed for the presence of selected seven phytochemicals (carbohydrate, protein, alkaloid, flavonoid, steroid, saponin, and tannin). The total phenolic and flavonoid contents of all of these medicinal plants were calculated. Asparagus officinalis (root) had the highest level of antioxidative activity suppression when all eight plants were tested against E. coli (DPPH assay: 86.70%). All of the aforementioned plants appear to have therapeutic potential, according to the available study. However, more investigation is certainly required to properly comprehend the potential relevance of medicinal plants found in urban green spaces.