This article discusses the pharmacognostic study on Tribulus terrestris and Withania somnifera. Fluorescence analysis and physical-chemical properties, such as ash and extractive values, have been performed. Additionally, preliminary phytochemical analysis and thin layer chromatographic behavior have been performed on the different extracts. Withania somnifera and Tribulus terrestris have been shown in research to have anti-inflammatory, anti-cancer, anti-parkinsonian, adaptogen, memory-enhancing, antioxidant, and anxiolytic properties. This article discusses the pharmacognostic study on Tribulus terrestris and Withania somnifera. Fluorescence analysis and physical-chemical properties, such as ash and extractive values, have been performed. Additionally, preliminary phytochemical analysis and thin layer chromatographic behavior have been performed on the different extracts. Withania somnifera and Tribulus terrestris have been shown in research to have anti-inflammatory, anti-cancer, anti-parkinsonian, adaptogen, memory-enhancing, antioxidant, and anxiolytic properties. Numerous other effects, such as immunomodulation, hypolipidemia, antibacterial, cardiovascular protection, sexual behavior, tolerance, and dependence have also been studied. These highly encouraging results call for further investigation of this plant to confirm these findings and clarify additional potential medicinal characteristics. Ashwagandha and Tribulus terrestris should be used in clinical research to treat a variety of diseases.