Medicinal plants are used for treatment because they have certain properties, including synergistic actions. The plants used in traditional medicine contain a vast variety of compounds and those compounds are used to treat chronic and infectious diseases. The active compounds in most parts of the medicinal plants have direct or indirect therapeutic effects and are used as medicinal agents. The present study revealed that the various alcoholic and aqueous extract of leaf of Vitex negundo linn and Vitex agnus castus linn contained the phytochemical constituents such as flavonoids, tannins, phenolics, sterols, carbohydrates, alkaloids and terpenoids which are the secondary metabolites of medicinal plant.