Moringa Oleifera plant exhibit many medicinal activities due to which it plays a significant role in herbal pharmaceuticals. The chemical phytoconstituents in the final extract of herbal medicines have a significant impact on their effectiveness. The conjugated approach Gas Chromatography-Mass Spectrometry (GC-MS) is used to pinpoint the chemical components in plant samples. This research work focused on extraction of compounds from bark of Moringa Oleifera plant belonging to family Moringaceae via Soxhlet extractor using solvents Benzene, Chloroform, Ethyl acetate and Hexane.The extract was subjects for GC-MS for detection of phytochemical constituents which play role in pharmaceutical sector. Phytochemical named dimethyl sulfoxide, tridecane, eicosane, dotriacontane, tetrapentacontane, n-hexadecanoic acid, 2,4-dimethyldodecane, 2,6,10-trimethyldodecane, heptadecane, heneicosane, dotriacontane and many more were detected from bark of M.Oleifera . More than 50 phytoconstituents were identified from M.Oleifera bark using four non polar solvents. Identified phytochemicals are associated with pharmacological activities.