Citrullus colocynthis and Trigonella foenum graceum are known in Ayurvedic medicine for their hypoglycemic and anti-diabetic properties. Traditional medicine involves the use of various plant extracts for phytochemical bioactive ingredients, which provide health benefits at an affordable price. Secondary metabolites are responsible for the medicinal activity of these plants. Method: Pharmacognosis study, physiochemical and qualitative phytochemical analysis of these plants, moisture content, pH, alcohol soluble extract, water soluble extract, total ash, acid soluble ash, water soluble ash, amino acids, Different test methods were used to find out. Phenols, tannins, flavonoids, proteins, sugar lowers, carbohydrates, lipids, saponins, alkaloids, glycosides, steroids and TLC. Result: The test confirms the presence of various parameters in physiochemicals and phytochemicals, mainly alkaloids, glycosides, flavonoids, carbohydrates, amino acids, proteins, bitter principles and saponins. Conclusion: The result shows the presence of phytochemical properties of these plants are bio-active component and responsible for these hypoglycemic and anti-diabetic effects in diabetic mice.