Plant sources are used as medicines for treating disease and disorder since from ancient times. They are widely used due to their safe and effective active principles and as they are clinically effective and less toxic. They produce an enormous amount of secondary metabolites (phytochemicals) with medicinal and antimicrobial properties. It has been documented that medicinal plants are used to cure diseases in the history of civilization. Sphagneticola trilobata is a plant species of family Asteraceae. It’s also called as Wedelia chinensis or Wedelia trilobata. It is a highly resistant, creeping perennial plant, with succulent stems. It has been found that Sphagneticola trilobata is used to treat various kinds of ailments including fever, bites and stings, cold, wounds, kidney dysfunction and dysmenorrhea. This research aims to study the phyto constituents, biological and therapeutic activities of Sphagneticola trilobata. This plant is effectively studied for its antimicrobial, anticancer, anti-inflammatory and anti-diabetic properties. The GC-MS analysis of Sphagneticola trilobata reveals that there are 15 bioactive compounds present in the methanolic leaf extract. Among those the first six bioactive compounds are evaluated for its antidiabetic properties