The present study focus on microscopy, phytochemical and morphological investigation of ethanolic extract of Caralluma adscendens (Roxb.) Bark. These plant shown various activity. Pharmaceutically valuable varieties of Caralluma adscendens from nodal explant, is described. The morphology of the Caralluma treated cells, control, and positive control were observed under reverse phase inverted microscope. , used by tribal Indians to suppress hunger and known as “famine food” with no history of adverse effects, which contains pregnane glycosides. This plant has been investigated for its myriad biological effects such as antihyperglycaemic and hypolipidaemic, hepatoprotective, antioxidant activity and came out with promising results. study of microscopy and phytochemical investigation take plant from hatti village, tahsil chandwad, district Nashik. Нese plants due to their several medicinal values are fast disappearing and are threatened to become rare due to indiscriminate collection and over exploitation by the pharmaceutical industry, agriculture, mining activities, and lopping for fodder used as an anti-obesity agent and appetite suppressor. It is also seen that the pregnane glycosides isolated and identified from African Hoodia are reported as anti-obesity and appetite-suppressant compounds. On reviewing the studies undertaken on the chemistry, pharmacology, and therapeutic potential of Caralluma is currently used as a “natural slimming” food supplement, likely due to its content in pregnane glycosides. In the present study, a commercially available