Seeds are the great sources of fibre. They contain healthy monounsaturated fats, polyunsaturated fats and many important vitamins, minerals, anti-inflammatory and antioxidants. In the present investigation, the phytochemical analysis and anti- inflammatory of Helianthus annuus L., (Sunflower) Ocimum basilicum L. (Basil) and Oryza sativa L. indica (Black rice) were carried out. Qualitative screening revealed the presence of alkaloids, glycosides, tannin and phenol, carbohydrate, saponin, anthocyanin, betacyanin, steroid, protein, coumarin, flavonoid, fat and oil. Among 16 phytochemical constituents, sunflower seed have 11 compounds when compared to basil seed and black rice. Sunflower seed are highly nutritious when compared to other two. In vitro anti-inflammatory assay of sunflower seed, basil seed and black rice extracts at different concentration were studied by protein denaturation. At 100mg concentration basil seed exhibit higher level of inhibition (48.3%) when compared to sunflower (47.9%) and black rice (46.6%). This suggests that seeds can be used as food supplements in every day diet for normal metabolic activities of living organism, to reduce the anti-nutritional effects and also useful in pharmaceutical industry.