Coastal sand dune plants are of great importance to researchers in the field of pharmacology, medicinal, industrial, nutritional, and ecosystem restoration. Nowadays most of the pharmaceutical industries depend on the medicinal plant for their raw materials for drug discovery. Spinifexlittoreus is a coastal plant under the family Poeaceae growing on the sandy seashore and is well known for its medicinal properties. The present study was carried out to the phytochemical screening and estimated the bioactive components present in the methanolicextract of Spinifexlittoreus leaves. The phytochemical studies revealed the presence of flavonoids, saponins, alkaloids, terpenoids, tannins, and phenols. The GC-MS analysis revealed the presence of twenty bioactive compounds which include n-Hexadecanoic acid, 9,10-Secochola-5,7,10(19)-trien-24-al, 3-hydroxy-(3.beta.,5Z,7E), and 1,3-Benzenedicarboxylic acid, etc., The phytochemical and GC-MS profiling of the methanol extract of Spinifexlittoreus leaves revealed the presence of bioactive compounds with important medicinal properties. Hence, the presence of these phytochemicals could be responsible for the therapeutic effects of the plant.