Human skin diseases, caused by dermatophytes, are common in North East India due to the high humid condition. The medicinal plants play the major role of treating the skin diseases in the region, especially among the ethnic people. The present study was undertaken to analyse different phytochemicals and biochemical properties of ten different medicinal plants used by traditional healers against dermatophytes. Based on the phytochemical constituents, four plants viz Dendrocnide sinuata (Blume) Chew, Meyna laxiflora Robyns, Sterculia villosa Roxb, and Eupotorium odoratum L were carried forward for further analyses. The result showed that the methanol, chloroform and aqueous leaf extracts of D. sinuata, M. laxiflora, S. villosa and E. odoratum hold promises as a source of pharmaceutically important phytochemicals like alkaloid, tannin, flavonoid and phenol. Methanol, chloroform and aqueous leaf extracts of M. laxiflora recorded higher content of phenol and flavonoid. Higher content of alkaloid was recorded in all the four selected plants. Total tannin content was found higher in E. odoratum followed by S. villosa, M. laxiflora and D. sinuata. Major biochemical compounds identified through GC-MS analysis are neophytadiene, linalool, indoles, terpenes, acetogenins, phenols, Z-28-Heptatriaconten-2-one, oxirane, hexadecyl, phytol, squalene and 2,4-DI-tert-butylphenol. These findings would be useful for further exploitation of the selected experimental plants for bioprospection.