Volume - 13 | Issue-1
Volume - 13 | Issue-1
Volume - 13 | Issue-1
Volume - 13 | Issue-1
Volume - 13 | Issue-1
The present study is aimed to analyse the chemical constituents of the chloroform, ethanol and petroleum ether extracts of leaves of Cassia tora through preliminary phytochemical investigation, HPLC and FTIR spectroscopy method. The results of the phytochemical study showed that flavonoids, alkaloids, saponins, glycosides, terpenoids, steroids, and tannins were present. With a C18-150 × 4.6 mm column, a 10 μinjection volume, and a 70:30 methanol: water mobile phase at 30°C, a reversed-phase HPLC analysis was carried out. The UV-detector at 254 nm was used to record the detection. The FTIR spectroscopic analyses of the extracts under study revealed varied characteristic stretching frequencies and peak values with various functional components. Preliminary phytochemical investigation of ethanol and petroleum ether leaves extract of Cassia tora confirmed the presence of emodin, anthraquinone, glycoside, tannin and alkaloids. The existence of primary amines, alcohols, amide, aldehydes, ethers, aromatics, alkanes, alkyl halides, alkenes, and aliphatic amines compounds, which display prominent peaks, was confirmed by FTIR analysis of leaf extracts in ethanol, chloroform, and petroleum ether. The phytochemical analysis, HPLC spectra, and FTIR spectrum profiles for these medicinally significant plants were produced as a consequence of the current study and can be applied in the field.