The search for natural antioxidants has gained considerable attention due to their potential health benefits and therapeutic applications. Phytochemical compounds derived from plants have gained significant attention due to their potential health benefits and therapeutic properties. In this study, we conducted a comprehensive phytochemical screening of two plant species, Gossypium barbadense (commonly known as cotton) and Nelumbo nucifera (also known as lotus). The study revealed the presence of diverse bioactive compounds, particularly flavonoids, phenols, and tannins, which contribute to free radical scavenging activity. Both the oils were evaluated for their total phenolic ccontent, total flavanoid content and free radical scavenging activity was carried out using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) method with ascorbic acid as reference standard and activity was extended for cell viability using human lung fibroblast (COPD), HCC7231 (TACC CCL-96).