Research is being done to determine whether or not traditional medicinal plants offer a viable alternative to the use of synthetic medicines in the treatment of diabetes. It is general knowledge that lemon oil and eucalyptus oil can be used as a treatment for a variety of diseases, including diabetes. This study was carried out with the intention of determining the in vitro effects of an ethanol extract of Eucalyptus citriodora (EOE) and lemon oil (LOE), as well as the possible mechanisms of action behind such effects. It was determined whether or whether BRIN BD11 clonal pancreatic -cells and mouse islets were capable of insulin secretion. The effects of EOE and LOE on glucose reduction were further investigated using a variety of additional in vitro models. Because of problems with bioavailability, organoleptic unacceptability, and other factors, the traditional ways of administering herbal medicine frequently result in noncompliance on the part of patients and a reduction in the effectiveness of the drug. This is true despite the immense potential of herbal therapy. It is important, while formulating dosage forms using eucalyptus bio-actives, to keep in mind the hypoglycemic potential of both eucalyptus and lemon. It is possible that the use of eucalyptus and lemon for the treatment of type 2 diabetes mellitus may gain greater momentum if nano-technological methods were utilized for their administration. This would lower the risks of adverse reactions, enhance their bioavailability, and improve their flavor. Additionally, it would improve their bioavailability