The Tephrosia villosa (L.) Pers herb extract were prepared by successive soxhlation i.e. extracting dried powder with the solvents of increasing order of polarity, and It was qualitatively observed that 70% ethanolic extract contain higher concentration of polyphenol, flavonoid, alkaloid, protein and tannin components andquantative studies of The total phenolic content of 70% EETVH was 1.08 mg/G expressed as equivalent to catechol. Similarly flavonoid content was found to be 3.21 mg/G expressed as equivalent to quercetin and total tannin content found to be 5.10 mg/G expressed as equivalent to tannic acid. Anti-diabetic property of the 70 % EETVH was studied in In-vitro and In-vivo models, . In-vitro non enzymatic glycosylation of haemoglobin method 70% EETVH significantely inhibited the hemoglobin glycosylation which is indicated by the presence of increasing concentration of hemoglobin, the plant extract exhibited higher inhibition indicating plant extract decreases the formation of glucose hemoglobin complex. In-vivo anti-diabetic activity of 70% EETVH is carried out in experimentally induced alloxan –diabetes in albino rats and to compare activity the lower dos( 1/10th cutoff value)and higher dose (1/5th) are selected from the toxicity studies according to OECD guidline no-423. It implies that 70% EETVH significantly reduces the elevated blood glucose