Wound healing is the replacement of damaged tissue via highly coordinated cellular events. The patient's condition, as well as the various types of wounds, further complicate the already complex healing process. Traditional wound dressing materials appear insufficient for facilitating and supporting this mechanism. Nanotechnology has the potential to provide the physicochemical properties and specific biological responses required to aid in the healing process. The Nano lipid is used for the delivery system to improve drug delivery and concentration in the site of action by reducing dosage form adverse reaction on skin healing and improve stability and loading capacity and prevent drug/active molecule expulsion during storage. Patients and Procedures: Prepare and standardize the structure Nano lipid by the solvent diffusion method, Seventy rabbits were divided into six groups a daily dressed treatment, the full excisional wounds were treated with three different formulas: conventional Honey&PDGF, Structural Nano Lipid (SNL- blank), and SNLHoney&PDGF gel. The linear wound healing rate t1/2, closure velocity, and effective concentrations (ECs20 and 50) of SNL in honey and PDGF were all measured. Results: The electron micrograph denoted the different size for honey 73.55± 1.04 nm, PDGF 51.92± 1.13 nm, SNLHoney 30.76 ±1.27 nm, SNLPDGF 142.50± 0.95 nm, and SNLHoney-PDGF 94.22± 0.77 nm respectively. As well as, the entrapment and loading efficiency was found the actual amount efficiency values, and the loading percent of SNLPDGF was 93.67 and 89.11% and SNLHoney was 91.19, and 87.99% respectively. While the absorbance curve showed distinctive main wavelength for SNLHoney& PDGF were 990.0, 230.5, and 196.0 nm also SNLHoney 992.0 and 228.0, SNLPDGF 989.5 and 225.0 nm compared to conventional honey and PDGF, which showed at 271.5 and 256.0 nm respectively, the XRD analysis SNLHoney-PDGF has sharp peaks and provided a particle size between 20 and 30 nm also SNLHoney 20–40nm, but SNLPDGF, honey, and PDGF are wider with lower intensities, negative Zeta potential values, however, the values to be considered stable in SNLHoney&PDGF, PDGF, and honey -18.5 mV, -14.0 mV, and -20.1 mV compared to SNLHoney and SNLPDGF -54.2 Mv and -61.8 mV and that demined the Nano-size lesser than that aggregated form, as well as, Fourier infrared spectroscopy showed the diagnostic functional peaks in both conventional and Nano form and mimic the chemical structure was not altered and preserved the chemical behavior properties. The wound healing results revealed that Nano lipid loaded Honey&PDGF gel reduced wounds and healed faster than conventional Honey&PDGF; higher closure rate, closure velocity, and low closure t1/2. The values of the ECS50 & 20 in SNL Honey and PDGF according to potency (EC50 SNLHoney 0.119, EC50 SNLPDGF 0.353, EC20 SNLHoney 0.129, and EC20 SNLPDGF 0.083%) wound closure time on the skin. As well as the tissue phenomics stereology appearance the scores of inflammatory decreased score values, decreased scab and vascularization score, increased the score of collagen fiber and extracellular matrix deposition of fibroblast, as well as, the epithelialization represented with increased time of wound healing increased epithelium significantly in SNLHoney-PDGF compared with other treated and control groups.