The main purpose of this study was to develop a topical delivery of Isoleucine to increase the therapeutic activity, to improve patient compliance and to avoid the side effects. Isoleucine is an aminoacid derivative resulting from reaction of Isoleucine at amino group to control blood sugar Topical gel formulations of Isoleucine were prepared by using carbopol 940 as a gelling agent with different concentrations. Five different formulations were prepared and evaluated with respect to color, spreadability, viscositymeasurement, determination of pH, drug content, Invitro drug release studies, and stability studies. Compatibility study was carried out by FT-IR spectral analysis. FT-IR study revealed that no interaction between drug and polymer. All the prepared formulations show acceptable physical properties. From the above observation it was concluded that this formulation could be a very promising topical substitute for treatment to control blood sugar