The management of dental caries revolves around prevention, control and removal of caries. Even after mechanical removal of caries, some part of infected dentin with viable bacteria remains hidden to the eyes of the clinician.Further, fluoride release of GIC is not sufficient to control the bacteria after loner clinical service. There is a need to device an effective material that had all advantages of GIC with additional antibacterial action. Aims: To evaluate “Linezolid loaded chitosan” nanoparticles, added to GIC and test for its physic(o)chemical and in vitro biological properties. Materials and Methods: Chitosan nanoparticles were synthesized and loaded with linezolid. Six different groups of GIC were prepared of which one was control group, that was devoid of any modifications. Five test groups were prepared by modifying the liquid. The GIC fluid was modified with 1, 2, 3, 4 and 5 wt% CH. Characterization was done by FTIR spectra, HPLC for release profile, Antibacterial activity studies using 3 bacteria and Invitro Cytotoxicity assays using human gingival fibroblast cells. Live/Dead assay was performed. Results: FTIR spectra showed successful incorporation of linezolid in chitosan. HPLC studies showed huge burst of release on the first day and lower amounts releasing on the 7th day. Antibacterial activity was present in all groups. Cytotoxicity assays showed that all modifications are biocompatible. and the preparation has great potential for use in the clinical scenario. Conclusion: The hypothesis that Linezolid, delivered through chitosan can offer prolonged antibacterial activity in the clinical service of GIC is accepted within the limitations of the study