Aim: The aim of the study was to develop a bi-phasic scaffold using the Chitosan, gelatin and BMP-2 protein and evaluation of the tensile strength and the scanning electron microscopic analysis of the scaffold. Materials and methods: The scaffold has been prepared by addition of 50 ml of acetic acid to 250 microlitre of De- ionized water to this solution 1 gram of Chitosan is added. The mixture of 5 grams of gelatin powder to 50 ml of deionized water and heating the solution in a 65 degree water bath for 30 minutes leading to dissolution of gelatin in the water. Then the solutions are mixed and then the gel is dried at 35 degrees for 24 hours for the scaffold formation. Then the scaffold is tested for the tea isle strength as well as the SEM analysis is done.