Background: Methicillin-resistant staphylococcus aureus (MRSA) infections have created big threats globally in the hospitals and communities due to their resistance with most of conventional antibiotics. To minimize infections of MRSA, continuous monitoring, knowledge of its prevalence and maintenance of hygienic condition have been required. Aim and objectives: To study the prevalence and molecular impacts of MRSA isolates with identification of MecA and ErmA genes in north Indian region. Methods: This was a cross sectional study performed in the Department of Microbiology and Central Research Laboratory of RMCH &RC (India) for a period of 3 years (Feb 2018 to Feb 2021). All the MRSA were collected mainly from blood and pus in different locations. All the standard phenotypic and genotypic protocols (disc diffusion, PCR etc) were followed to perform this study. Results: A total of 90 MRSA isolates were identified by CX, OX, and E-test. The prevalence of MRSA was found to be around 41% in our study. MRSA isolates were found more in pus samples (61.11%) with the maximum age of 41-50 years. All MRSA isolates were found sensitive to linezolid, Teicoplanin, vancomycin, and Gentamycin however all the isolates were recorded resistant with Cefoxitin and Oxacillin. The presence of MecA gene was recorded in all the 90 isolates of MRSA while the presence of ErmA gene was found in 5 isolates only. The presence of MecA and ErmA gene was confirmed by the PCR followed by sequencing.