Background: Chronic fatigue is a pervasive health concern affecting individuals worldwide, significantly impacting their daily lives. While systemic illnesses are often linked to chronic fatigue, here is the increasing interest in understanding the potential role of serum vitamin deficiency in contributing to the current condition. This study focuses on investigating occurrence of serum vitamin deficiency in patients experiencing chronic fatigue in Pakistan, a region where the impact of nutritional factors on health is significant. Aim: The primary purpose of our current research is to assess occurrence of serum vitamin deficiency among individuals in Pakistan who suffer from chronic fatigue without any apparent systemic illness. By examining the nutritional status of this specific population, the study aims to shed light on potential connection among vitamin deficiencies and chronic fatigue. Methods: The study will involve a cohort of 300 participants, carefully selected from the local population in Pakistan. The duration of the study is set at one year, ensuring a comprehensive evaluation of the participants' serum vitamin levels. The research will take place at Ha'il University in Saudi Arabia, providing a controlled and conducive environment for data collection. Participants will undergo thorough clinical assessments to exclude any underlying systemic illnesses contributing to their fatigue. Blood samples will be collected to analyze the serum levels of essential vitamins, including but not limited to Vitamin D, Vitamin B12, and folate. Additionally, participants will be required to provide detailed information about their dietary habits, lifestyle, and any supplementation they may be taking. Results: Upon completion of the study, the results will offer important insights into prevalence of serum vitamin deficiency among individuals experiencing chronic fatigue in Pakistan. The data will be studied by means of statistical approaches to determine correlations between specific vitamin deficiencies and the severity or duration of fatigue. The findings will contribute to present body of knowledge on potential role of nutritional factors in the manifestation of chronic fatigue. Conclusion: Our current research aims to contribute to our understanding of connection among serum vitamin deficiency and chronic fatigue in absence of systemic illnesses. The results may have implications for public health interventions, emphasizing the importance of nutritional assessments in individuals with chronic fatigue. If a significant association is found, it could pave the way for targeted interventions, such as dietary modifications or vitamin supplementation, to alleviate the burden of chronic fatigue in this population.