Probiotics foods are those which contain micro-organisms and influence the consumer’s health by regulating microbial balance in the intestine. The prebiotics are selectively utilized by host microorganisms as a substrate and significantly improve metabolic activity, enhancing digestion, nutrient absorption ability and the immune system. The term synbiotics refers to the ingredients which contain both pre and probiotics. It will enhance the viability of microrganisms against the high pH of GI tract as well as facilitate absorption of bioactive components by the body Probiotics, prebiotics, and synbiotics supplementation maintains the optimal microflora in the gut and also helps in the prevention of numerous diseases. In this review article the isolation of probiotics, mechanism of action and health benefits are discussed briefly which will help in futuristic research.