The industrial prospective of alkaline protease hold extensive application in various industries like tannery, detergent, food and leather industries. In the present study 25 different Bacterial species were isolated from garden soil, Pollachi, Coimbatore. From this potential isolates were screened for their potential against gram positive and gram negative organisms. The isolate showing the property of alkaline protease was then identified by 16S RNA sequencing and species were identified as Bacillus australimaris. The isolate was cultivated in submerged fermentation in Horikoshi -I (alkaline medium) and further enhanced by optimization of cultural parameters (pH, temperature, Carbon, Nitrogen, Aeration and Agitation). The maximum alkaline protease enzyme production from this isolate were observed at pH 9 at 50°C. Ammonium sulphate and Glucose are designated a good source for alkaline protease production by Bacillus australimaris. A Bacillus auralimatis has been used for optimal production through media standardization and appropriate purification methods for produce an alkaline protease. Molecular weight was found as 25 kDa. Following 48 h of incubation, a protease production profiling study indicated a maximum enzyme activity (147.9 U/ml).The enzyme extract was immobilized using calcium alginate for industrial application.