Antibiotic resistance has escalated into a serious worldwide concern. Antibiotic-resistant infections and microorganisms in the environment are heavily influenced by hotspot habitats. This study used a selective growth medium to isolate, characterize and profile various antibiotic-resistant bacteria found in hotspot environments of the Khammam district. The selective growth medium contained bacterial isolates at various concentrations of Ampicillin, Amoxicillin clavulanate, Cefuroxime, Ceftriaxone, Imipenem, Gentamycin, Nalidixic Acid, Ciprofloxacin, Erythromycin and Tetracycline.