Background: Gallstone disease is a prevalent condition that can lead to complications, including cholecystitis, pancreatitis, and cholangitis. Gallstones are classified into three main types: cholesterol stones, pigment stones, and mixed stones. The histopathological changes in the gallbladder mucosa have been associated with the type of gallstone; however, the relationship between these factors remains controversial. So, The aim of this study investigates the association between the histopathological changes in the gallbladder mucosa and the type of gallstone. Methodology: Cross-Sectional study was conducted in the Department of Pathology in collaboration with the Department of Surgery. A total of 150 patients who underwent cholecystectomy for symptomatic gallstone disease were included in the study. Study didn't include specimens with no clinical information, autolyzed samples, acalculous cholecystitis, or having already been diagnosed with cancer. The collection period of specimens was one year. The samples were collected after obtaining informed consent and with the approval of the institutional review board. Logistic Regression Analysis, T test and Chi square test are applied for analysis. Results: Cholesterol stones were the most common type of gallstone, followed by mixed and pigment stones. Chronic cholecystitis was the most common histopathological change observed, followed by cholesterolosis, fibrosis, and pigment deposition. The frequency of chronic cholecystitis and cholesterolosis was significantly higher in patients with cholesterol stones than those with mixed or pigment stones.