Lead acetate (PbAc) is a widespread form of global toxicity in addition to low level exposure of lead acetate induce neurotoxic and neurobehavioral changes. The current study was carried out to investigate the valuable properties of Eugenol in protecting the lead induced neurotoxicity in rats. Toxicity created by lead acetate in groups II, III, and IV rats. The toxic effect of lead is confirmed by using antioxidant assay, liver and renal functional test. Antioxidants enzymes are capable of improving free radicals before going to scavenge the cells. Additionally, PbAc administration significantly decreased the antioxidant enzymes like, GSH, GR, GPX, SOD& CAT and increased level of LPO. Moreover, PbAc induced neurotoxic which was evident that due to significant increase in the serum markers level of AST, ALT, ALP, total protein, albumin and bilirubin in liver. Urea and Creatinine in renal markers. Treatment with Eugenol at a dose of 250mg/kg b.wt, mixed with olive oil considerably shows good activity for all the enzymatic antioxidants. These outcomes indicate the neuroprotective activity and antioxidant of eugenol and it attests that it has a defensive role against the oxidative stress caused by lead acetate in rats.