Giant gourami (Osphronemus goramy), a giant air-breathing freshwater fish that is a good candidate for freshwater aquaculture due to its high price, high local demand and good meat quality. This study aimed to determine the growth and survival rate of Giant gourami (Osphronemus goramy) fed different protein diet sources. It was conducted in a freshwater concrete tanks for 60 days with three corresponding treatments: T0 – Commercial Feeds (CF), T1 – Mulberry Leaves (ML), and T2 –Chicken Intestine (CI) replicated thrice. Growth increment (GI) in terms of weight, length, and width, specific growth rate (SGR), and survival rate (SR) were measured and data were analyzed using Kruskal Wallis Test at α=0.05. Results demonstrated that giant gourami fed with CI got the highest GI and SGR. In terms of survival rate, all of the treatments got 100% SR. Results showed that all treatments significantly affect the growth increment (weight, length, width) and specific growth rate but did not significantly affect the survival rate of giant gourami. In addition, giant gourami fed with chicken intestine resulted significantly the best growth among all treatments and can be recommended to be an alternative feed better growth in giant gourami aquaculture.