Understanding the complex interactions between plants and microbes in the rhizosphere is vital for improving agricultural productivity and promoting sustainable crop production. To unravel theseinteractions, a comprehensive approach incorporating OMICS techniques such as proteome and secretome analysis is employed. Proteome analysis, which examines the complete set of proteins expressed by an organism, provides valuable insights into the molecular mechanisms underlying plant- microbe interactions in the rhizosphere. This environment serves as a critical habitat where plants and microorganisms interact, influencing each other's growth and functionality. Advanced techniques like one and two-dimensional electrophoresis, difference gel electrophoresis (DIGE), highly sensitive mass spectrometry (MS), and isotope labeling methods such as isobaric tags for relative and absolute quantification (iTRAQ) are utilized to analyze and compare protein expression profiles. Another significant aspect of proteome analysis in plant-microbe interactions is the study of the secretome. The secretome refers to the proteins released by microorganisms into the extracellular environment. These secreted proteins play essential roles in host recognition, colonization, and pathogenicity. By examining the secretome, researchers can gain insights into the virulence factors and mechanisms of pathogenicity employed by microorganisms. Techniques like vacuum infiltration centrifugation (VIC) and gravity- extraction method (GEM) are employed to isolate secreted proteins for analysis.