Volume - 13 | Issue-1
Volume - 13 | Issue-1
Volume - 13 | Issue-1
Volume - 13 | Issue-1
Volume - 13 | Issue-1
This work is concerned with the method development and validation of sildenafil citrate as an active pharmaceutical ingredient by HPTLC (high-performance thin-layer chromatographic) technique by using hydrotropic agents. The pre-coated silica gel 60 F254 aluminum plate was selected as the stationary phase, and the solvent system consisted of 10 % sodium benzoate, 10 % sodium acetate, 3.5 % citric acid (5 : 3 : 2 v/v/v) was used as developing solvents. Analysis of sildenafil citrate was carried out at 295 nm and being detected at an Rf of 0.72. The developed method was validated for various parameters such as linearity, accuracy, precision, limit of detection (LOD), limit of quantitation (LOQ), and robustness. The correlation coefficient of sildenafil citrate was found to be 0.996. The calibration plot was linear between 500-1000 ug/mL, respectively. The average percentage recovery of sildenafil citrate was found to be 99.83 %. Intra and inter-day precision measured as RSD was less than 2%.