Taverniera is a Fabaceae genus with twelve species indigenous to Northeast African and Southwestern Asian nations (Stadler et al. 1994). The herb Taverniera cuneifolia (Roth) Arn., also known as Indian licorice, grows along the banks of tiny streams. Plant growth regulators are substances, not nutrients, that encourage and impact improved growth and development in little doses. Auxins (indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), -naphthalene acetic acid (NAA), 2, 4-dichlorophenoxy-acetic acid (2, 4-D) and cytokinins (Benzylaminopurine (BAP), Kinetin (Kn), 2-isopentyladenine (2-iP), Thidiazuron (TDZ) and auxin for the best outcomes, BAP was used alone or in conjunction with Kin, IBA, IAA, or NAA used as shoot preparation from meristem. The use of plant cell cultures to manufacture secondary metabolites has been a popular method of utilizing plants' biosynthetic capacity to produce desired secondary metabolites. Routian and Nickell (1956) were the first to get a patent for plant tissue culture-based substance synthesis. In callus, suspension cultures, and hairy root cultures, several researchers have reported the generation of valuable chemicals. It can be concluded that Glycyrrhiza acid is widely studied around the world but is limited to extraction by a single plant, licorice roots (Glycyrrhiza glabra). However, the current investigation of Taverniera cunerifolia for GA was successfully carried out using the HPTLC method and correlated with the standard component. The findings of the study can be used to better understand the plant's numerous phytochemical features and to establish long-term extraction of the component for a variety of medical and industrial applications.