Kidney Transplantation, although the most cost-effective and preferred treatment in patients with end-stage renal disease, is limited by severe shortage of cadaver kidneys. To ameliorate this limitation, use of living donors is now widely accepted, in particular because it results in better recipient and renal graft survival. It has been shown that transplantation survival for kidneys from living donors, is higher than cadaveric sources (80% vs. 67% at 5 years). Aim & Objective: 1. Radiological study of role of multi-detector CT in the pre-operative work up of potential renal donors. 2.To determine the accuracy of multi-detector CT as the primary imaging technique in the evaluation of living kidney donor. Methods: Study design: Prospective Observational study. Study setting: Department of Radio-diagnosis, Bombay Hospital, Mumbai. Study duration: From June 2012 to May 2014 (2 years). Study population: A total of 30 potential donors were selected for study using consecutive type of non-probability Sampling. Sample size: 30. Results: Mean Age of the study subjects was 47.7 ± 9.9 years. Two third of the patients belonged to age group of 41 to 60 years. 70% of the donors were females while 30% were males. in Right renal artery. Out of total, 80% subjects had single artery (63.3% single while 16.7% had different variations) while remaining 20% had dual renal arteries with different variations. Out of total 83.3% subjects (63.3% single while 20% had different variations) had single artery while remaining 16.7% had dual renal arteries with different variations. Most of the subjects (96.7%) were donors of left sided Kidney while only 3.3% were right kidney donors. Conclusions: Most of donors were female, Most subjects were left Kidney donors, Compared with arteriography and urography, MDCT is less invasive. More important, for pre-surgical planning, MDCT provides highly accurate imaging of the renal collecting and vascular systems, especially in revealing vascular variations