Used cooking oil components can contain various chemical compounds due to uncontrolled heating and repeated frying in various types of food. In making biodiesel, used cooking oil needs to be purified to improve the quality of the results by using moringa seed powder and charcoal and moringa seed powder activated with nitric acid. The purpose of this study was to analyze biodiesel from the results of refining used cooking oil using moringa seeds in the form of powder, charcoal and powder moringa seeds activated with nitric acid. The results of refining used cooking oil using moringa seeds showed a qualitative change in pH so that it is good to use as a biodiesel raw material. The quality of biodiesel from proximate results such as volatile compounds, water content, ash, and fixed carbon is in accordance with SNI. The analysis of volatile compounds was followed by GCMS analysis. The percentage of volatile compounds by proximate increases the acquisition of volatile compounds but the ester compounds formed by GCMS are less than 96% using used cooking oil raw materials refined with Moringa seed powder, this result is best from charcoal and Moringa seed powder activated with nitric acid.