Silymarin (SN) is a lipophilic polyphenol flavonoid, isolated from the Silybum marianum L. Geartn (milk thistle) which belongs to the family Aster of Asteraceae or Compositae. Silymarin has anti-oxidative, antidiabetic, antifibrotic, anti-inflammatory and cytoprotective, hepatoprotector, immunomodulator properties. In the current research the renoprotective effect of Silymarin (SN) in Wistar rats against gentamicin induced kidney damage was studied. The results revealed that the Gentamicin (80 mg/kg, i.p.) treated rats body weight of was significantly lower than the CMC suspension treated rats. In rats the SN (100 mg/kg, p.o.) treated has shows increased in the body weight, in the biochemical estimation, gentamicin alone treated rats, showed significant decrease in total protein content and an increase in serum creatinine, serum urea, serum uric acid, and blood urea nitrogen (BUN) as compared to control rats, SN along with gentamicin treated rats resulted in a significant increase in total protein content and a decrease in serum creatinine, urea, uricacid, and BUN. In histopathological examination, compared to gentamicin-treated rats, SN decreased the severity of the gentamicin-induced renal necrotic damage by reducing the histopathological damages.