The current study aims to examine the effect of Alhagi graecorum ethanolic extract on intestinal function and histopathological changes in male rabbits. The experiment employed twenty male rabbits. The animals were equally separated into four groups and given treatment for 42 days as follows: control group (C): Rabbits were allowed free supply to drinking water, T1 group: Animals received (10 mg /kg b.w) of aspirin orally, T2 group: were administered 400 mg/ kg b.w ethanolic extract of Alhagi graecorum and 10 mg/kg of aspirin orally , T3 group: animals were given 400 mg/ kg b.w alcoholic extract of Alhagi graecorum orally. blood samples collected at the end of the experiment from fasting animals to assess serum tumor necrosis factor alpha (TNF-α) and Citrulline levels. Immediately after animals were scarified, small piece from the duodenum was collected for assessment of Occludin protein (ocln gene) expression, small intestine (duodenum) was removed for histopathological examination. The results shown that oral administration of Aspirin for 42 days caused intestinal damage manifested by a substantial decline (P<0.05) in ocln gene and serum level of citrulline in the T1group, whereas the animals given Alhagi graecorum showed a considerable elevation (P<0.05) in the levels of ocln gene and citrulline. While serum level of TNF-α significantly (P<0.05) elevated in T1 group comparison with other groups. The histopathological examination revealed pathological changes in aspirin group while giving alhagi graecorum alcoholic extract with and without aspirin was effective in modified these changes into semi normal. The alcoholic extract of Alhagi graecorum improved intestinal functions and protected intestinal tissue from damage induced by Aspirin.