Women experience hormonal changes in their life time, in addition, comorbidities also may affect them. Diabetes, hypertension, obesity, dyslipidemia dominate in postmenopausal period and several inflammatory markers, female specific risk factors influence risk of Atherosclerotic Cardiovascular disease (ASCVD) at postmenopausal age. Though a few studies have been carried out on selected biochemical markers of CVD in postmenopausal women with comorbidities, no detailed research has so far been focused on women in pre and perimenopausal stages. In this study, 210 women in the age group of 20-65 years who attended the Gynecology Out- Patient Department of a tertiary care hospital were recruited and stratified into pre, peri and postmenopausal groups (70 subjects in each group) based on STRAW classification. Those women with comorbidities, blood was collected and and biochemical markers such as high sensitivity C-reactive protein (hs-CRP), interleukin-6 (IL-6) and fibrinogen besides homocysteine (Hcy), lipid parameters, Apolipoprotein-B and estradiol were estimated. From among 70 subjects in each of pre, peri and postmenopausal groups, women with comorbidities were 27%, 28% and 43% respectively. Significant Higher total cholesterol, triglycerides, LDL-C and HDL-C found in peri and postmenopausal women with comorbidities. There was significant elevation in homocysteine (p<0.000), fibrinogen (p<0.05) IL-6 and hs-CRP in comorbid postmenopausal women with comorbidities when compared to pre and peri groups. Hyperhomocystienemia (HHcy) and higher hs-CRP levels found in peri and postmenopausal women. Hence an early screening of these biochemical markers to identify cardiac risk and aggressive risk factor lowering strategies with positive lifestyle patterns in peri and postmenopausal women are suggesting together was recommended along with appropriate management to overcome the development of ASCVD in their later age.