This review article aims to explore the crucial role of nurses in promoting adherence to laboratory testing protocols among patients with chronic conditions. Laboratory testing plays a vital role in the management of chronic diseases, as it provides valuable information for diagnosis, monitoring disease progression, and evaluating treatment efficacy. However, non-adherence to laboratory testing protocols among patients with chronic conditions remains a significant challenge that can lead to adverse health outcomes. Nurses, as frontline healthcare providers, play a key role in promoting adherence to these protocols through patient education, counseling, and ongoing support. The review will examine the current literature on the barriers to adherence to laboratory testing protocols among patients with chronic conditions and the various strategies that nurses can employ to overcome these barriers. These strategies may include patient education on the importance of regular testing, addressing misconceptions and fears related to testing procedures, providing emotional support, and utilizing technology to facilitate testing reminders and follow-up. Furthermore, the review will highlight the impact of nurse-led interventions on improving adherence to laboratory testing protocols and ultimately enhancing patient outcomes. By empowering nurses with the knowledge and skills to effectively promote adherence to testing protocols, healthcare organizations can improve the quality of care provided to patients with chronic conditions and optimize disease management. Overall, this review aims to underscore the critical role that nurses play in promoting adherence to laboratory testing protocols among patients with chronic conditions and to provide insights into effective strategies for enhancing patient compliance with testing recommendations.