Background: Rigid thoracoscopy is the gold standard tool for diagnosing exudative pleural effusion, but sometimes it is difficult to obtain sufficient biopsies using the conventional rigid forceps biopsy (RFB). This study evaluated the efficacy, safety, and diagnostic value of a new technique using rigid cryoprobe to obtain pleural biopsies during rigid thoracoscopy. Methods. In a single-center, interventional, prospective study, patients with exudative undiagnosed pleural effusion were evaluated with a rigid forceps biopsy pleuroscopy followed by a cryoprobe at the same setting between April 2022 to March 2023.All biopsies were processed for histopathology examination by an independent pathologist; any complications were recorded. Results: A total of 22 patients (median age 63.95years) were included in the study, most of were men (54.5%). Both RFB and CPB established definitive diagnoses in 20/22 (90.9%) of cases. The predominant histopathological examination in patients was metastatic malignancy (45.45%): followed by mesothelioma (31.8%). The sample size (largest length in (mm) Mean ±SD) obtained by CPB (8.9±1.2mm) was significantly larger than RFB (5.1±1.02) (P < 0.0001). Crushed cells (artifacts) prevalence in biopsy there was a statistically significant decrease in CPB in comparison with RFB(P=0.006). There was a significant decrease when comparing between two studied biopsies regarding bleeding at biopsy bed in favor to CPB (P=0.031). There were no significant complications or procedure-related deaths. Conclusion: Cryobiopsies obtained during medical thoracoscopy are technically feasible and safe with high diagnostic value. Biopsies of cryoprobe were larger than that of rigid forceps, and with better preserved cellular architecture. These results will encourage the use of cryotechnique for diagnosis of undiagnosed exudative pleural effusion