It is well known fact that complete edentulism causes a deficiency of multiple macro and micronutrients. Vitamin D is one of the important micronutrients which needs to be evaluated in such patients. This study was done to evaluate role of micronutrient Vitamin D on the muscle function and muscle tonicity through serum estimations, ultrasonography, electromyography and questioner-based analysis before and after therapy. Materials and Method: An interventional study was conducted at Dr Z.A Dental College AMU Aligarh and was supported by ICMR. A total of 130 patients without any teeth aged between 40-70 years were selected for the research and on the basis of serum Vitamin D levels were categorized into groups A & B. Group A (Control group) and Group B (Supplemented with Vitamin D drug): Patients were given Complete dentures of both group and were evaluated using EMG & USG. ResultsVitamin D quantities varied from 9.5 to 38.5 ng/ml in the concerned samples.