Diarrheal disease is a major concern in developing world with 248 million episodes every year. 453,000 children die from rotavirus diarrhoea every year and India alone accounts for 22% of deaths. This study was to determine Rotavirus A infection among immunized children less than 5 years. A total of 323 stool samples were collected to determine the presence of Rotavirus antigen and conformation was done through Rotavirus A through RT-PCR. Results showed a prevalence 44.3% of Rotavirus A infection among immunized children. Infection was high among the age group of 6-12 months followed by 13-24 months and lowest among 0-6 months.