Tianeptine is used as an antidepressant agent. A new rapid, simple, Economical and environmental friendly High-Performance Thin-Layer Chromatography (HPTLC) has been developed and validated for quantitative determination of Tianeptine in marketed formulation. HPTLC separation was performed on aluminium plates precoated with silica gel 60 RP-18F-254S as the stationary phase using Chloroform: Methanol (90:10% v/v) as mobile phase. Quantification was achieved by densitometric analysis at 210nm over the concentration range of 200–1200 ng/band. The method was found to give compact and well resolved band for Tianeptine at Retention factor (Rf) 0.70± 0.74. The linear regression analysis data for calibration graph showed good linear relationship with r2 = 0.999. The method was validated for precision, recovery, robustness, ruggedness and sensitivity as per International conference on Harmonization (ICH) guidelines. The Limit of Detection (LOD) and Limit of Quantification (LOQ) were found to be 5.23 ng and 15.85 ng, respectively. The proposed developed RP-HPTLC method can be applied for identification and quantitative determination of Tianeptine in marketed formulation